Introduction: Electroporation has become a routine technique for rapid drug delivery for the treatment of cancer. Because of its simplicity and wide range of application, it has been applied for the transfer of gold-nanoparticles and can facilitate entry into target cancer cells. The aim of this study is finding optimal conditions in order to obtain high GNPs- uptake and cell viability by means of electroporation.Materials and Methods: Exponential electrical pulse with electric field intensity ranging from 0.2 -2 kV/cm, pulse length of 100 Âµs and the pulse number of 2 was used. Electroporated cell viability was investigated using MTS assay and GNPs-cellular uptake was assayed using graphite furnace atomic absorption spectrometry (GFAAS). Finally, electroporation results were compared with passive method.Results and Conclusion: The maximum uptake occurred at 1.2 to 2 kV/cm and passive method happened. The cell viability of 1.2 kV/cm and passive method was about 90%, while the cell viability in 2 kV/cm drastically decreased to 50%. The findings showed that using two pulses of 1.2 kV/cm and 100 Âµs is a convenient way and surrogate of passive method for internalizing GNPs into cells.