TY - JOUR ID - 47375 TI - A β-Amyloid(1-42) Biosensor Based on Molecularly Imprinted Poly-Pyrrole for Early Diagnosis of Alzheimer’s Disease JO - Journal of Biomedical Physics and Engineering JA - JBPE LA - en SN - AU - Dehdari Vais, Rezvan AU - Yadegari, Hossein AU - Heli, Hossein AU - Sattarahmady, Naghmeh AD - PhD Candidate, Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran AD - PhD, Department of Mechanical and Materials Engineering, University of Western Ontario, London, Ontario N6A 5B9, Canada AD - PhD, Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Y1 - 2021 PY - 2021 VL - 11 IS - 2 SP - 215 EP - 228 KW - Beta-Amyloid KW - β-Amyloid (1-40) KW - Molecular Imprinting KW - Artificial Antibody KW - Neurodegenerative Disease KW - Sensory Aids KW - Biosensing Technique KW - Bioprobe DO - 10.31661/jbpe.v0i0.1070 N2 - Background: Alzheimer’s disease (AD) is a common form of dementia, characterized by production and deposition of β-amyloid peptide in the brain. Thus, β-amyloid peptide is a potentially promising biomarker used to diagnose and monitor the progression of AD. Objective: The study aims to develop a biosensor based on a molecularly imprinted poly-pyrrole for detection of β-amyloid.Material and Methods: In this experimental study, an imprinted poly-pyrrole was employed as an artificial receptor synthesized by electro-polymerization of pyrrole on screen-printed carbon electrodes in the presence of β-amyloid. β-amyloid acts as a molecular template within the polymer. The biosensor was evaluated by cyclic voltammetry using ferro/ferricyanide marker. The parameters influencing the biosensor performance, including electro-polymerization cycle umbers and β-amyloid binding time were optimized to achieve the best biosensor sensitivity. Results: The β-amyloid binding affinity with the biosensor surface was evaluated by the Freundlich isotherm, and Freundlich constant and exponent were obtained as 0.22 ng mL-1 and 10.60, respectively. The biosensor demonstrated a detection limit of 1.2 pg mL-1. The biosensor was applied for β-amyloid determination in artificial cerebrospinal fluid. Conclusion: The biosensor is applicable for early Alzheimer’s disease detection. UR - https://jbpe.sums.ac.ir/article_47375.html L1 - https://jbpe.sums.ac.ir/article_47375_d78e362360728230db4ad66839e0c3f7.pdf ER -