Journal of Biomedical Physics and Engineering

An Aptamer-based Biosensor for Troponin I Detection in Diagnosis of Myocardial Infarction

Document Type : Original Research

Authors

M Negahdary 1, 2
M Behjati-Ardakani 1
N Sattarahmady 2, 3
H Heli 2

1 Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

2 Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

3 Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

https://doi.org/10.31661/jbpe.v8i2.930
Abstract
Background: Acute myocardial infarction (MI) accounts for one third of deaths. Cardiac troponin I (TnI) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of MI.Objectives: In this study, a molecular method is introduced to early diagnosis of MI by rapid detection of TnI.Materials and Methods: The detection method was based on electrochemical aptasensing, being developed using different methods and evaluation steps. A gold electrode was used as a transducer to successful immobilize 76base aptamer to fabricate a TnI biosensor.Results: The designed aptasensor could detect TnI in a range of 0.03 to 2.0 ng mL-1 without using any label, pre-concentration or amplification steps. The limit of detection was attained as 10 pg mL-1 without significant trouble of interfering species. The TnI biosensor demonestrated a stable, regenerative and reproducible function. 89 human samples were used to evaluate the performance of the TnI biosensor, and it represented 100% and 81%, diagnostic sensitivity and specificity, respectively.Conclusions: This aptasensor may be used as an applicable tool in the future of early medical diagnosis of MI.

Keywords

Electrochemical Biosensor
Biomarker
Heart Failure
Troponin
Molecular Detection
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Journal of Biomedical Physics and Engineering
Volume 8, Issue 2 - Serial Number 2
28
Spring 2018
Pages 167-178

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