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An Aptamer-based Biosensor for Troponin I Detection in Diagnosis of Myocardial Infarction

Document Type : Original Research

Authors

1 Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

2 Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

3 Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Background: Acute myocardial infarction (MI) accounts for one third of deaths. Cardiac troponin I (TnI) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of MI.Objectives: In this study, a molecular method is introduced to early diagnosis of MI by rapid detection of TnI.Materials and Methods: The detection method was based on electrochemical aptasensing, being developed using different methods and evaluation steps. A gold electrode was used as a transducer to successful immobilize 76base aptamer to fabricate a TnI biosensor.Results: The designed aptasensor could detect TnI in a range of 0.03 to 2.0 ng mL-1 without using any label, pre-concentration or amplification steps. The limit of detection was attained as 10 pg mL-1 without significant trouble of interfering species. The TnI biosensor demonestrated a stable, regenerative and reproducible function. 89 human samples were used to evaluate the performance of the TnI biosensor, and it represented 100% and 81%, diagnostic sensitivity and specificity, respectively.Conclusions: This aptasensor may be used as an applicable tool in the future of early medical diagnosis of MI.

Keywords

References
  1. Newman AB, Arnold AM, Naydeck BL, Fried LP, Burke GL, Enright P, et al. “Successful aging”: effect of subclinical cardiovascular disease. Arch Intern Med. 2003;163:2315-22. doi: 10.1001/archinte.163.19.2315. PubMed PMID: 14581251.
  2. Taylor J. Third universal definition of myocardial infarction. Eur Heart J. 2012;33:2506-7. doi: 10.1093/eurheartj/ehs296. PubMed PMID: 23065972.
  3. Norhammar A, Johansson I, Thrainsdottir IS, Rydén L. Congestive heart failure. Textbook of Diabetes. 2017:659-72.
  4. Vargas-Barron J, Antunez-Montes O-Y, Roldán F-J, Aranda-Frausto A, González-Pacheco H, Romero-Cardenas Á, et al. Myocardial rupture in acute myocardial infarction: Mechanistic explanation based on the ventricular myocardial band hypothesis. Rev Invest Clin. 2015;67:318-22.
  5. Arevalo HJ, Vadakkumpadan F, Guallar E, Jebb A, Malamas P, Wu KC, et al. Arrhythmia risk stratification of patients after myocardial infarction using personalized heart models. Nat Commun. 2016;7:11437. doi: 10.1038/ncomms11437. PubMed PMID: 27164184; PubMed Central PMCID: PMC4866040.
  6. LeWinter MM. Clinical practice. Acute pericarditis. N Engl J Med. 2014;371:2410-6. doi: 10.1056/NEJMcp1404070. PubMed PMID: 25517707.
  7. hiele H, Zeymer U, Neumann FJ, Ferenc M, Olbrich HG, Hausleiter J, et al. Intra-aortic balloon counterpulsation in acute myocardial infarction complicated by cardiogenic shock (IABP-SHOCK II): final 12 month results of a randomised, open-label trial. Lancet. 2013;382:1638-45. doi: 10.1016/S0140-6736(13)61783-3. PubMed PMID: 24011548.
  8. Moy E, Barrett M, Coffey R, Hines AL, Newman-Toker DE. Missed diagnoses of acute myocardial infarction in the emergency department: variation by patient and facility characteristics. Diagnosis (Berl). 2015;2:29-40. doi: 10.1515/dx-2014-0053. PubMed PMID: 29540019.
  9. Chan D, Ng LL. Biomarkers in acute myocardial infarction. BMC Med. 2010;8:34. doi: 10.1186/1741-7015-8-34. PubMed PMID: 20529285; PubMed Central PMCID: PMC2898678.
  10. Natsukawa T, Maeda N, Fukuda S, Yamaoka M, Fujishima Y, Nagao H, et al. Significant Association of Serum Adiponectin and Creatine Kinase-MB Levels in ST-Segment Elevation Myocardial Infarction. J Atheroscler Thromb. 2017;24:793-803. doi: 10.5551/jat.38232. PubMed PMID: 28100880; PubMed Central PMCID: PMC5556187.
  11. Yokoyama M, Watanabe T, Otaki Y, Takahashi H, Arimoto T, Shishido T, et al. Association of the Aspartate Aminotransferase to Alanine Aminotransferase Ratio with BNP Level and Cardiovascular Mortality in the General Population: The Yamagata Study 10-Year Follow-Up. Dis Markers. 2016;2016:4857917. doi: 10.1155/2016/4857917. PubMed PMID: 27872510; PubMed Central PMCID: PMC5107869.
  12. Buckner SL, Loenneke JP, Loprinzi PD. Cross-Sectional Association Between Normal-Range Lactate Dehydrogenase, Physical Activity and Cardiovascular Disease Risk Score. Sports Med. 2016;46:467-72. doi: 10.1007/s40279-015-0457-x. PubMed PMID: 26694048.
  13. Mair J, Thygesen K. Using Cardiac Troponins in Patients with Acute Myocardial Infarction. Cardiac Biomarkers: Springer; 2016. p. 57-68.
  14. Negahdary M, Behjati-Ardakani M, Sattarahmady N, Yadegari H, Heli H. Electrochemical aptasensing of human cardiac troponin I based on an array of gold nanodumbbells-Applied to early detection of myocardial infarction. Sensors and Actuators B: Chemical. 2017;252:62-71. doi: 10.1016/j.snb.2017.05.149.
  15. Apple FS, Collinson PO, Biomarkers ITFoCAoC. Analytical characteristics of high-sensitivity cardiac troponin assays. Clin Chem. 2012;58:54-61. doi: 10.1373/clinchem.2011.165795. PubMed PMID: 21965555.
  16. Apple FS, Wu AH. Myocardial infarction redefined: role of cardiac troponin testing. Clin Chem. 2001;47:377-9. PubMed PMID: 11238285.
  17. Apple FS, Pearce LA, Smith SW, Kaczmarek JM, Murakami MM. Role of monitoring changes in sensitive cardiac troponin I assay results for early diagnosis of myocardial infarction and prediction of risk of adverse events. Clin Chem. 2009;55:930-7. doi: 10.1373/clinchem.2008.114728. PubMed PMID: 19299542.
  18. Roppolo LP, Fitzgerald R, Dillow J, Ziegler T, Rice M, Maisel A. A comparison of troponin T and troponin I as predictors of cardiac events in patients undergoing chronic dialysis at a Veteran’s Hospital: a pilot study. J Am Coll Cardiol. 1999;34:448-54. PubMed PMID: 10440158.
  19. Heidenreich PA, Alloggiamento T, Melsop K, McDonald KM, Go AS, Hlatky MA. The prognostic value of troponin in patients with non-ST elevation acute coronary syndromes: a meta-analysis. J Am Coll Cardiol. 2001;38:478-85. PubMed PMID: 11499741.
  20. Khan NA, Hemmelgarn BR, Tonelli M, Thompson CR, Levin A. Prognostic value of troponin T and I among asymptomatic patients with end-stage renal disease: a meta-analysis. Circulation. 2005;112:3088-96. doi: 10.1161/CIRCULATIONAHA.105.560128. PubMed PMID: 16286604.
  21. Liu X, Wei J, Song D, Zhang Z, Zhang H, Luo G. Determination of affinities and antigenic epitopes of bovine cardiac troponin I (cTnI) with monoclonal antibodies by surface plasmon resonance biosensor. Anal Biochem. 2003;314:301-9. PubMed PMID: 12654317.
  22. Li F, Yu Y, Cui H, Yang D, Bian Z. Label-free electrochemiluminescence immunosensor for cardiac troponin I using luminol functionalized gold nanoparticles as a sensing platform. Analyst. 2013;138:1844-50. doi: 10.1039/c3an36805j. PubMed PMID: 23377497.
  23. Mondello C, Cardia L, Ventura-Spagnolo E. Immunohistochemical detection of early myocardial infarction: a systematic review. Int J Legal Med. 2017;131:411-21. doi: 10.1007/s00414-016-1494-1. PubMed PMID: 27885432.
  24. Zhang X, Zhang X, Lei M, Lin Y, Megson IL, Wei J, et al. Detection of circulating IgG antibodies to apolipoprotein B100 in acute myocardial infarction. FEBS Open Bio. 2015;5:712-6. doi: 10.1016/j.fob.2015.08.006. PubMed PMID: 26425439; PubMed Central PMCID: PMC4564368.
  25. Grant SA, Pierce ME, Lichlyter DJ, Grant DA. Effects of immobilization on a FRET immunosensor for the detection of myocardial infarction. Anal Bioanal Chem. 2005;381:1012-8. doi: 10.1007/s00216-004-2976-4. PubMed PMID: 15696275.
  26. Kong T, Su R, Zhang B, Zhang Q, Cheng G. CMOS-compatible, label-free silicon-nanowire biosensors to detect cardiac troponin I for acute myocardial infarction diagnosis. Biosens Bioelectron. 2012;34:267-72. doi: 10.1016/j.bios.2012.02.019. PubMed PMID: 22386490.
  27. Liu Y, Lipponen K, Cilpa-Karhu G, Oorni K, Riekkola ML. Plasma low-density lipoprotein immobilized silica as stationary phase in nano-liquid chromatography. J Chromatogr A. 2012;1270:104-10. doi: 10.1016/j.chroma.2012.10.049. PubMed PMID: 23159197.
  28. Islam MS, Kang SH. Chemiluminescence detection of label-free C-reactive protein based on catalytic activity of gold nanoparticles. Talanta. 2011;84:752-8. doi: 10.1016/j.talanta.2011.02.001. PubMed PMID: 21482278.
  29. Qureshi A, Gurbuz Y, Niazi JH. Biosensors for cardiac biomarkers detection: a review. Sensors and Actuators B: Chemical. 2012;171:62-76. doi: 10.1016/j.snb.2012.05.077.
  30. Sattarahmady N, Rahi A, Heli H. A signal-on built in-marker electrochemical aptasensor for human prostate-specific antigen based on a hairbrush-like gold nanostructure. Sci Rep. 2017;7:11238. doi: 10.1038/s41598-017-11680-5. PubMed PMID: 28894225; PubMed Central PMCID: PMC5593896.
  31. Mohammadi J, Moattari A, Sattarahmady N, Pirbonyeh N, Yadegari H, Heli H. Electrochemical biosensing of influenza A subtype genome based on meso/macroporous cobalt (II) oxide nanoflakes-applied to human samples. Anal Chim Acta. 2017;979:51-7. doi: 10.1016/j.aca.2017.05.010. PubMed PMID: 28599709.
  32. Sattarahmady N, Kayani Z, Heli H. Highly simple and visual colorimetric detection of Brucella melitensis genomic DNA in clinical samples based on gold nanoparticles. Journal of the Iranian Chemical Society. 2015;12:1569-76. doi: 10.1007/s13738-015-0629-5.
  33. Moradi M, Sattarahmady N, Rahi A, Hatam GR, Sorkhabadi SMR, Heli H. A label-free, PCR-free and signal-on electrochemical DNA biosensor for Leishmania major based on gold nanoleaves. Talanta. 2016;161:48-53. doi: 10.1016/j.talanta.2016.08.030. PubMed PMID: 27769435.
  34. Mayer G. Nucleic Acid and Peptide Aptamers: Methods and Protocols. New York: Humana Press; 2014.
  35. Heli H, Sattarahmady N, Zare SN. Electrooxidation and determination of perphenazine on a graphene oxide nanosheet-modified electrode. RSC Adv. 2015;5:21005-11. doi: 10.1039/C5RA01405K.
  36. Sattarahmady N, Heli H. An electrocatalytic transducer for L-cysteine detection based on cobalt hexacyanoferrate nanoparticles with a core-shell structure. Anal Biochem. 2011;409:74-80. doi: 10.1016/j.ab.2010.09.032. PubMed PMID: 20869939.
  37. Heli H. A study of double stranded DNA adsorption on aluminum surface by means of electrochemical impedance spectroscopy. Colloids Surf B Biointerfaces. 2014;116:526-30. doi: 10.1016/j.colsurfb.2014.01.046. PubMed PMID: 24576822.
  38. Heli H, Faramarzi F, Sattarahmady N. Oxidation and determination of Gabapentin on nanotubes of nickel oxide-modified carbon paste electrode. Journal of Solid State Electrochemistry. 2012;16:45-52.
  39. Heli H, Sattarahmady N, Hajjizadeh M. Electrocatalytic oxidation and electrochemical detection of guanine, l-arginine and l-lysine at a copper nanoparticles-modified electrode. Analytical Methods. 2014;6:6981-9. doi: 10.1039/C4AY01507J.
Journal of Biomedical Physics and Engineering
Volume 8, Issue 2 - Serial Number 2
28
June 2018
Pages 167-178
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