Document Type : Original Research

Authors

• Zh Ghorbani ¹
• R Fardid ^{2, 3}
• G H Haddadi ^{2, 3}
• A Derakhshanfar ⁴
• A Kouhpayeh ⁵
• Z Haddadi ⁶

¹ MSc, Radiology Department, School of Paramedicine, Shiraz University of Medical Sciences, Shiraz, Iran

² PhD, Radiology Department, School of Paramedicine, Shiraz University of Medical Sciences, Shiraz, Iran

³ PhD, Ionizing and Non-ionizing Radiation Protection Research Center (INIRPRC), School of Paramedicine, Shiraz University of Medical Sciences, Shiraz, Iran

⁴ PhD, Diagnostic Laboratory Sciences and Technology Research Center, Basic Sciences in Infection Diseases Research Center, Center of Comparative & Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

⁵ PhD, Department of Pharmacology, Fasa University of Medical Science, Fasa, Iran

⁶ MD, Medical student, Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran

Abstract

Background: Hesperidin is a bioflavonoid glycoside mainly found in citrus fruit and has been shown radio-protective potential in various measurement systems.
Objective: In this article aims to investigate the radio-protective effect of hesperidin on the liver of Sprague Dawely rats.
Material and Methods: In this clinical study, 40 male rats were selected randomly and divided into 8 groups. Group 1 did not receive radiation and hesperidin (sham control). Group 2 received only 100 mg/kg body weight (b.w) of hesperidin for 7 consecutive days (HES group); group 3 exposed to dose of 2Gy whole body gamma radiation (2Gy group), and group 4 and 5 received 50 and 100 mg/kg b.w of HES for 7 consecutive days before 2 Gy gamma radiation, respectively.Group 6 exposed to dose of 8Gy gamma radiation (8Gy group); group 7 and 8 received 50 and 100 mg/kg b.w of HES for 7 days before 8Gy gamma irradiation, respectively. Histopathological evaluation was perfomred 24 hours after radiation.
Results: Administration of hesperidin (50 mg/kg b.w, 7 days) before 2Gy of gamma irradiation led to remove inflammatory mononuclear cells in the portal space. Microscopic findings in the groups receiving two doses of hesperidin (50 and 100 mg/kg b.w, orally, 7 days), before 8Gy of gamma radiation, were similar in a way that extreme dilation of central veins to be seen, however, there was no capillarization.
Conclusion: HES can be offered as a suitable radio-protector in radiotherapy patients and radiation workers.

Keywords

• Gamma Rays
• Hesperidin
• Radio-Protector
• Hyperemia
• Capillarization