Journal of Biomedical Physics and Engineering

Magnetic Resonance Imaging Property of Doxorubicin-Loaded Gadolinium/13X Zeolite/Folic Acid Nanocomposite

Document Type : Original Research

Authors

S Ghaderi 1, 2
B Divband 3, 4
N Gharehaghaji 5

1 MSc, Medical Radiation Sciences Research Team, Tabriz University of Medical Sciences, Tabriz, Iran

2 MSc, Department of Radiology, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran

3 PhD, Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

4 PhD, Inorganic Chemistry Department, Faculty of Chemistry, University of Tabriz, Tabriz, Iran

5 PhD, Department of Radiology, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran

https://doi.org/10.31661/jbpe.v0i0.1254
Abstract
Background: Magnetic resonance imaging (MRI) using nanostructures has been a proper method for tumor targeting purposes. Different MRI nanomaterials, targeting agents and anticancer drugs have been used for targeting of tumors.
Objectives: This study aims to consider the MRI property of doxorubicin (DOX)-loaded gadolinium/13X zeolite/folic acid (Gd3+/13X/FA) nanocomposite.
Material and Methods: In this in vitro study, Gd3+/13X/FA/DOX nanocomposite was prepared and the X-ray diffraction, scanning electron microscopy and MTT assay were conducted to evaluate the physicochemical properties of the nanocomposite. MRI was performed at 25°C using a 1.5 T clinical system to determine the T1 relaxation times and subsequently, the T1 relaxivity.
Results: The size of the nanocomposite was in the range of 80-200 nm. The nanocomposite without DOX loading (Gd3+/13X/FA) showed compatibility for A549 cells for all concentrations while DOX-loaded nanocomposite was toxic for 62% of the cells at the concentration of 0.4 mg/ml. The T1 relaxivity of Gd3+/13X/FA/DOX nanocomposite was 4.0401 mM-1s-1.
Conclusion: Gd3+/13X/FA/DOX nanocomposite shows a T1 relaxivity similar to the conventional gadolinium chelates, and a successful DOX loading.

Keywords

Magnetic Resonance Imaging
Relaxivity
Gadolinium
Doxorubicin
Folic Acid
13X zeolite
  1. Oghabian MA, Guiti M, Haddad P, Gharehaghaji N, Saber R, Alam NR, et al. Detection sensitivity of MRI using ultra-small super paramagnetic iron oxide nano-particles (USPIO) in biological tissues. Conf Proc IEEE Eng Med Biol Soc. 2006;5625-6. doi: 10.1109/IEMBS.2006.260131. PubMed PMID: 17945909.
  2. Lutz AM, Weishaupt D, Persohn E, Goepfert K, Froehlich J, Sasse B, et al. Imaging of macrophages in soft-tissue infection in rats: relationship between ultrasmall superparamagnetic iron oxide dose and MR signal characteristics. Radiology. 2005;234(3):765-75. doi: 10.1148/radiol.2343031172. PubMed PMID: 15665219.
  3. Saharkhiz H, Gharehaghaji N, Nazarpoor M, Mesbahi A, Pourissa M. The Effect of Inversion Time on the Relationship Between Iron Oxide Nanoparticles Concentration and Signal Intensity in T1-Weighted MR Images. Iran J Radiol. 2014;11(2):e12667. doi: 10.5812/iranjradiol.12667. PubMed PMID: 25035696. PubMed PMCID: PMC4090637.
  4. Ren L, Chen S, Li H, Zhang Z, Zhong J, Liu M, et al. MRI-guided liposomes for targeted tandem chemotherapy and therapeutic response prediction. Acta Biomater. 2016;35:260-8. doi: 10.1016/j.actbio.2016.02.011. PubMed PMID: 26873364.
  5. Ma Z, Wan H, Wang W, Zhang X, Uno T, Yang Q, et al. A theranostic agent for cancer therapy and imaging in the second near-infrared window. Nano Research. 2019;12(2):273-9. doi: 10.1007/s12274-018-2210-x. PubMed PMID: 31832124. PubMed PMCID: PMC6907162.
  6. Inozemtseva OA, German SV, Navolokin NA, Bucharskaya AB, Maslyakova GN, Gorin DA. Encapsulated magnetite nanoparticles: preparation and application as multifunctional tool for drug delivery systems. Nanotechnology and Biosensors. 2018;175-192. doi: 10.1016/B978-0-12-813855-7.00006-4.
  7. Stephen ZR, Kievit FM, Zhang M. Magnetite nanoparticles for medical MR imaging. Mater Today (Kidlington). 2011;14:330-8. doi: 10.1016/S1369-7021(11)70163-8. PubMed PMID: 22389583. PubMed PMCID: PMC3290401
  8. Yang CT, Padmanabhan P, Gulyás BZ. Gadolinium (iii) based nanoparticles for T 1-weighted magnetic resonance imaging probes. RSC Advances. 2016;6(65):60945-66. doi: 10.1039/C6RA07782J.
  9. Lin YS, Hung Y, Su JK, Lee R, Chang C, Lin ML, et al. Gadolinium (III)-incorporated nanosized mesoporous silica as potential magnetic resonance imaging contrast agents. The Journal of Physical Chemistry B. 2004;108(40):15608-11. doi: 10.1021/jp047829a.
  10. Sun C, Sze R, Zhang M. Folic acid-PEG conjugated superparamagnetic nanoparticles for targeted cellular uptake and detection by MRI. J Biomed Mater Res A. 2006;78(3):550-7. doi: 10.1002/jbm.a.30781. PubMed PMID: 16736484.
  11. Stella B, Arpicco S, Peracchia MT, Desmaële D, Hoebeke J, Renoir M, et al. Design of folic acid-conjugated nanoparticles for drug targeting. J Pharm Sci. 2000;89(11):1452-64. dio: 10.1002/1520-6017(200011)89:113.0.co;2-p. PubMed PMID: 11015690.
  12. Babayevska N, Florczak P, Woźniak-Budych M, Jarek M, Nowaczyk G, Zalewski T, et al. Functionalized multimodal ZnO@Gd2O3 nanosystems to use as perspective contrast agent for MRI. Applied Surface Science. 2017;404:129-37. doi: 10.1016/j.apsusc.2017.01.274.
  13. Shen X, Li T, Chen Z, Geng Y, Xie X, Li S, et al. Luminescent/magnetic PLGA-based hybrid nanocomposites: a smart nanocarrier system for targeted codelivery and dual-modality imaging in cancer theranostics. Int J Nanomedicine. 2017;12:4299. doi: 10.2147/IJN.S136766. PubMed PMID: 28652734. PubMed PMCID: PMC5473604.
  14. Fernández M, Javaid F, Chudasama V. Advances in targeting the folate receptor in the treatment/imaging of cancers. Chem Sci. 2018;9(4):790-810. doi: 10.1039/C7SC04004K. PubMed PMID: 29675145.PubMed PMCID: PMC5890329.
  15. Zhang G, Gao J, Qian J, Zhang L, Zheng K, Zhong K, et al. Hydroxylated mesoporous nanosilica coated by polyethylenimine coupled with gadolinium and folic acid: a tumor-targeted T1 magnetic resonance contrast agent and drug delivery system. ACS Appl Mater Interfaces. 2015;7(26):14192-200. doi: 10.1021/acsami.5b04294. PubMed PMID: 26084052.
  16. Shin J, Jo D, Hong SB. Rediscovery of the importance of inorganic synthesis parameters in the search for new zeolites. AAcc Chem Res. 2019;52(5):1419-1427. doi: 10.1021/acs.accounts.9b00073. PubMed PMID: 31013053.
  17. Li Y, Li L, Yu J. Applications of zeolites in sustainable chemistry. Chem. 2017;3(6):928-49. doi: 10.1016/j.chempr.2017.10.009.
  18. Bresinska I, Balkus KJ. Studies of Gd (III)-exchanged Y-type zeolites relevant to magnetic resonance imaging. J Phys Chem. 1994;98(49):12989-94. doi: 10.1021/j100100a029.
  19. Csajbók É, Bányai I, Vander Elst L, Muller RN, Zhou W, Peters JA. Gadolinium (III)-loaded nanoparticulate zeolites as potential high-field MRI contrast agents: Relationship between structure and relaxivity. Chemistry. 2005;11(16):4799-807. doi: 10.1002/chem.200500039. PubMed PMID: 15929138.
  20. Mesgari-Shadi A, Sarrafzadeh MH, Divband B, Barar J, Omidi Y. Batch adsorption/desorption for purification of scfv antibodies using nanozeolite microspheres. Microporous and Mesoporous Materials. 2018;264:167-75. doi: 10.1016/j.micromeso.2018.01.028.
  21. Anbia M, Aghaei M. Study of the effect of organic binders on 13X zeolite agglomeration and their CO2 adsorption properties. Scientia Iranica. 2019;26(3):1497-504. doi: 10.24200/sci.2018.21198.
Journal of Biomedical Physics and Engineering
Volume 10, Issue 1
37
February 2020
Pages 103-110

Article View 45,601
PDF Download 114,698

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