Gold-Curcumin Nanostructure in Photo-thermal Therapy on Breast Cancer Cell Line: 650 and 808 nm Diode Lasers as Light Sources

Rahimi-Moghaddam, F; Sattarahmady, N; Azarpira, N

doi:10.31661/jbpe.v0i0.906

Gold-Curcumin Nanostructure in Photo-thermal Therapy on Breast Cancer Cell Line: 650 and 808 nm Diode Lasers as Light Sources

Document Type : Original Research

Authors

F Rahimi-Moghaddam ¹

N Sattarahmady ¹^{, 2}

N Azarpira ³

¹ Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

² Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

³ Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

https://doi.org/10.31661/jbpe.v0i0.906

Abstract

Background: Au nanoparticles (AuNPs) exhibit very unique physiochemical and optical properties, which now are extensively studied in a range of medical diagnostic and therapeutic applications. AuNPs can be used for cancer clinical treatment with minimal invasion. On the other hand, curcumin is a polyphenol derived from turmeric which is used for medical purposes due to its anti-cancer, anti-microbial, anti-oxidant and anti-inflammatory properties. Despite these potential properties of curcumin, its usage is limited in medicine due to low solubility in water. Conjugation of curcumin to AuNPs (Au-Cur nanostructure) can be increasing its solubility. Photo-thermal therapy (PTT) is a novel kind of cancer treatment which involves two major components: laser and photo-thermoconversion agents.
Materials and Methods: Here, diode lasers emitting 808 nm and 650 nm were utilized as light sources, and synthesized Au-Cur nanostructure was applied as a photo-thermo conversion agent. UV-vis absorbance spectroscopy and dynamic light scattering (DLS) were applied to study the maximum absorption of particles, size stability of the samples and their zeta potential. The synthesized Au-Cur nanostructure under irradiation of laser is used for PPT on 4T1 cells. The cytotoxicity activity of Au-Cur nanostructure and laser irradiation on 4T1 cells was evaluated by MTT assay.
Results: Synthesized Au-Cur nanostructure showed λ_max at 540 nm and a mean hydrodynamic diameter of 25.8 nm. 4T1 cells were exposed to an 808 nm diode laser (1.5 W cm², 10 min) in the presence of different concentrations of Au-Cur nanostructure. Next, 4T1 cells with Au-Vur nanostructure were exposed to diode laser beam (650 nm, 1.5 W cm²) for 10 min. The results revealed that Au-Cur nanostructure under laser irradiation of 808 nm more decreased cell viability of 4T1 cells compared to laser irradiation of 650 nm.
Conclusion: It was concluded that combining an 808-nm laser at a power density of 1.5W/cm² with Au-Cur nanostructure has a destruction effect on 4T1 breast cancer cells in vitro experiments compared to laser irradiation of 650 nm.

Keywords

Curcumin

Lasers

Semiconductor

Nanostructures

Farajzadeh R, Pilehvar-Soltanahmadi Y, Dadashpour M, Javidfar S, Lotfi-Attari J, Sadeghzadeh H, et al. Nano-encapsulated metformin-curcumin in PLGA/PEG inhibits synergistically growth and hTERT gene expression in human breast cancer cells. Artif Cells Nanomed Biotechnol. 2017:1-9. doi: 10.1080/21691401.2017.1347879. PubMed PMID: 28678551.
Sharma H, Mishra PK, Talegaonkar S, Vaidya B. Metal nanoparticles: a theranostic nanotool against cancer. Drug Discov Today. 2015;20:1143-51. doi: 10.1016/j.drudis.2015.05.009. PubMed PMID: 26007605.
Mirkin CA, Meade TJ, Petrosko SH, Stegh AH. Nanotechnology-based precision tools for the detection and treatment of cancer: Springer; 2015.
Rao W, Zhang W, Poventud-Fuentes I, Wang Y, Lei Y, Agarwal P, et al. Thermally responsive nanoparticle-encapsulated curcumin and its combination with mild hyperthermia for enhanced cancer cell destruction. Acta Biomater. 2014;10:831-42. PubMed PMID: 24516867; PubMed Central PMCID: PMC4136765.
Lindner U, Weersink RA, Haider MA, Gertner MR, Davidson SR, Atri M, et al. Image guided photothermal focal therapy for localized prostate cancer: phase I trial. J Urol. 2009;182:1371-7. doi: 10.1016/j.juro.2009.06.035. PubMed PMID: 19683262.
Betrouni N, Colin P, Bozzini G, Mordon S. Image-guided laser therapies for prostate cancer. IRBM. 2013;34:28-32.
Shi J, Votruba AR, Farokhzad OC, Langer R. Nanotechnology in drug delivery and tissue engineering: from discovery to applications. Nano Lett. 2010;10:3223-30. doi: 10.1021/nl102184c. PubMed PMID: 20726522; PubMed Central PMCID: PMC2935937.
Riley RS, Day ES. Gold nanoparticle-mediated photothermal therapy: applications and opportunities for multimodal cancer treatment. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2017;9. doi: 10.1002/wnan.1449. PubMed PMID: 28160445; PubMed Central PMCID: PMC5474189.
Abo-Elfadl MT, Gamal-Eldeen AM, Elshafey MM, Abdalla GM, Ali SS, Ali MR, et al. Photothermal therapeutic effect of PEGylated gold nano-semicubes in chemically-induced skin cancer in mice. J Photochem Photobiol B. 2016;164:21-9. doi: 10.1016/j.jphotobiol.2016.09.012. PubMed PMID: 27636008.
Jiang BP, Zhang L, Guo XL, Shen XC, Wang Y, Zhu Y, et al. Poly(N-phenylglycine)-Based Nanoparticles as Highly Effective and Targeted Near-Infrared Photothermal Therapy/Photodynamic Therapeutic Agents for Malignant Melanoma. Small. 2017;13. doi: 10.1002/smll.201602496. PubMed PMID: 27982516.
Gobin AM, Lee MH, Halas NJ, James WD, Drezek RA, West JL. Near-infrared resonant nanoshells for combined optical imaging and photothermal cancer therapy. Nano Lett. 2007;7:1929-34. doi: 10.1021/nl070610y. PubMed PMID: 17550297.
Mousavy SJ, Riazi GH, Kamarei M, Aliakbarian H, Sattarahmady N, Sharifizadeh A, et al. Effects of mobile phone radiofrequency on the structure and function of the normal human hemoglobin. Int J Biol Macromol. 2009;44:278-85. PubMed PMID: 19263507.
Shibu ES, Hamada M, Murase N, Biju V. Nanomaterials formulations for photothermal and photodynamic therapy of cancer. Journal of Photochemistry and Photobiology C: Photochemistry Reviews. 2013;15:53-72.
Darne PA, Mehta MR, Agawane SB, Prabhune AA. Bioavailability studies of curcumin–sophorolipid nano-conjugates in the aqueous phase: role in the synthesis of uniform gold nanoparticles. RSC Advances. 2016;6:68504-14.
Pari L, Tewas D, Eckel J. Role of curcumin in health and disease. Arch Physiol Biochem. 2008;114:127-49. doi: 10.1080/13813450802033958. PubMed PMID: 18484280.
Lopez-Lazaro M. Anticancer and carcinogenic properties of curcumin: considerations for its clinical development as a cancer chemopreventive and chemotherapeutic agent. Mol Nutr Food Res. 2008;52 Suppl 1:S103-27. doi: 10.1002/mnfr.200700238. PubMed PMID: 18496811.
Sindhu K, Rajaram A, Sreeram K, Rajaram R. curcumin conjugated gold nanoparticle synthesis and its biocompatibility. Rsc Advances. 2014;4:1808-
rar SK, Verma M. Measurement of nanoparticles by light-scattering techniques. TrAC Trends in Analytical Chemistry. 2011;30:4-17.
Lotya M, Rakovich A, Donegan JF, Coleman JN. Measuring the lateral size of liquid-exfoliated nanosheets with dynamic light scattering. Nanotechnology. 2013;24:265703. doi: 10.1088/0957-4484/24/26/265703. PubMed PMID: 23732310.
Arjmandi N, Van Roy W, Lagae L, Borghs G. Measuring the electric charge and zeta potential of nanometer-sized objects using pyramidal-shaped nanopores. Anal Chem. 2012;84:8490-6. doi: 10.1021/ac300705z. PubMed PMID: 22901005.
Chen C, Johnston TD, Jeon H, Gedaly R, McHugh PP, Burke TG, et al. An in vitro study of liposomal curcumin: stability, toxicity and biological activity in human lymphocytes and Epstein-Barr virus-transformed human B-cells. Int J Pharm. 2009;366:133-9. doi: 10.1016/j.ijpharm.2008.09.009. PubMed PMID: 18840516.
Zeng X, Leung A, Xia X, Yu H, Bai D, Xiang J, et al. Effect of blue light radiation on curcumin-induced cell death of breast cancer cells. Laser Physics. 2010;20:1500-3.
Dovigo LN, Pavarina AC, Ribeiro AP, Brunetti IL, Costa CA, Jacomassi DP, et al. Investigation of the photodynamic effects of curcumin against Candida albicans. Photochem Photobiol. 2011;87:895-903. doi: 10.1111/j.1751-1097.2011.00937.x. PubMed PMID: 21517888.
Andrade MC, Ribeiro AP, Dovigo LN, Brunetti IL, Giampaolo ET, Bagnato VS, et al. Effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of Candida spp. Arch Oral Biol. 2013;58:200-10. doi: 10.1016/j.archoralbio.2012.10.011. PubMed: 23153629.
Karmakar S, Banik NL, Ray SK. curcumin suppressed anti-apoptotic signals and activated cysteine proteases for apoptosis in human malignant glioblastoma U87MG cells. Neurochem Res. 2007;32:2103-13. doi: 10.1007/s11064-007-9376-z. PubMed PMID: 17562168.
Heidari M, Sattarahmady N, Azarpira N, Heli H, Mehdizadeh AR, Zare T. Photothermal cancer therapy by gold-ferrite nanocomposite and near-infrared laser in animal model. Lasers Med Sci. 2016;31:221-7. doi: 10.1007/s10103-015-1847-x. PubMed PMID: 26694488.
Lencioni R, Cioni D, Crocetti L, Franchini C, Pina CD, Lera J, et al. Early-stage hepatocellular carcinoma in patients with cirrhosis: long-term results of percutaneous image-guided radiofrequency ablation. Radiology. 2005;234:961-7. doi: 10.1148/radiol.2343040350. PubMed PMID: 15665226.
Liang P, Dong B, Yu X, Yu D, Wang Y, Feng L, et al. Prognostic factors for survival in patients with hepatocellular carcinoma after percutaneous microwave ablation. Radiology. 2005;235:299-307. doi: 10.1148/radiol.2351031944. PubMed PMID: 15731369.
Hinshaw JL, Lee FT, Jr. Cryoablation for liver cancer. Tech Vasc Interv Radiol. 2007;10:47-57. doi: 10.1053/j.tvir.2007.08.005. PubMed PMID: 17980318.
Robinson JT, Welsher K, Tabakman SM, Sherlock SP, Wang H, Luong R, et al. High Performance In Vivo Near-IR (>1 mum) Imaging and Photothermal Cancer Therapy with Carbon Nanotubes. Nano Res. 2010;3:779-93. doi: 10.1007/s12274-010-0045-1. PubMed PMID: 21804931; PubMed Central PMCID: PMC3143483.