Document Type : Original Research
Authors
- Seyed Mohammad Javad Mortazavi 1, 2
- Fatemeh Shekoohi Shooli 1, 3
- Fatemeh Kadivar 1
- Shahrokh Zare 4
- Abolfazel Kanani 1
- Mohammad Amin Mosleh-Shirazi 1
- Sadegh Masoudi 1
- Feridoun Karimi-Busheri 5
- Davood Mehrabani 4, 5, 6, 7
1 Ionizing and Non-Ionizing Radiation Protection Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2 Fox Chase Cancer Center, Philadelphia, PA 19111, USA
3 Institute for Advanced Biomedical Technologies, Via Luigi Polacchi, 11, 66100 Chieti CH, Italy
4 Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
5 Department of Oncology, Cross Cancer Institute, University of Alberta, Edmonton, Alberta, Canada
6 Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
7 Comparative and Experimental Medicine Center, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract
Background: Following any injury in radiation accidents, such as Acute Radiation Syndrome (ARS), medical interventions are recommended based on radiation dose and physiological response. Clinical management encompasses blood transfusion, hematopoietic cytokines, and stem-cell transplantation.
Objective: This study aimed to determine the effect of adipose tissue-derived stem cells (AdSCs) together with vitamin C on the survival of rats with ARS.
Material and Methods: In this experimental study, 45 rats were randomly divided into 3 equal groups of animals administered with a single dose of oral 400 mg/kg vitamin C; those injected intravenously with 1.5×105 AdSCs; and rats transplanted intravenously with 1.5×105 AdSCs together with a single oral dose of 400 mg/kg vitamin C. All rats were already irradiated with 10 Gy (dose rate 0.286 Gy/ min) 60CO, for 35 minutes with a field size of 35 cm × 35 cm for all body areas.
Results: A significant increase in survival rate was visible one month after γ irradiation in 73.3% of animals received AdSCs+vitamin C, 60% of rats injected with just AdSCs, and 13.3% of the group received just vitamin C.
Conclusion: Our findings revealed significant efficacy of a combined approach involving AdSC transplantation and vitamin C to enhance the survival rate following lethal irradiation. This combination could offer a potential avenue for addressing the alleviation of tissue damage caused by chemotherapy and toxic drugs. We recommend the administration of AdSCs together with vitamin C as an effective and prompt treatment option for irradiation injuries.
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