Document Type : Original Research
Authors
- Farid Mortazavi 1, 2
- Paria Tamaddon 2
- Ali Ketabi 1, 3
- Hanieh Haghighi 1
- Kiana Khajeheian 2
- Masoud Haghani 1, 4
- Tahereh Mahmoudi 1, 2
- Sadegh Masjoodi 5
- Masoud Negahdary 6, 7
- Naghmeh Sattarahmady 1, 2
1 Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2 Department of Medical Physics and Engineering, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
3 Physics Unit, Department of Radio-oncology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4 Department of Radiology, School of Paramedical Science, Shiraz University of Medical Sciences, Shiraz, Iran
5 Neuroscience Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
6 Department of Biomedical Engineering, Texas A&M University, 600 Discovery Drive, College Station, TX, 77840-3006, USA
7 Center for Remote Health Technologies & Systems, Texas A&M Engineering Experiment Station, 600 Discovery Drive, College Station, TX, 77840-3006, USA
Abstract
Background: Lung cancer is a leading cause of cancer-related mortality worldwide, underscoring the need for the development of more effective treatment strategies. Radiotherapy (RT), particularly intensity-modulated radiation therapy (IMRT), has enhanced tumor targeting while minimizing damage to healthy tissues. Nevertheless, radioresistance and challenges posed by the tumor microenvironment limit its efficacy.
Objective: Selenium-curcumin-polyethylene glycol 600 nanoparticles (Se-Cur-PEG NPs) analyzed as radiosensitizers in IMRT for lung cancer treatment.
Material and Methods: In this experimental study, Se-Cur-PEG NPs were synthesized and characterized for their potential as radiosensitizers.
Results: The in vitro toxicity of Se-Cur-PEG NPs against A549 lung cancer cells was evaluated using MTT assays, demonstrating a dose-dependent reduction in cell viability. The combination of Se-Cur-PEG NPs (50 µg mL-1) with IMRT (4 Gy) resulted in a significant enhancement in cell death compared to either treatment alone, indicating a strong synergistic effect (CI=1.21) and a notable sensitizer enhancement ratio (SER=2.5). Intracellular ROS generation analysis confirmed that Se-Cur-PEG NPs amplified IMRT-induced oxidative stress, contributing to increased cancer cell toxicity.
Conclusion: These findings suggest that Se-Cur-PEG NPs hold promise as effective radiosensitizers, potentially improving lung cancer RT outcomes.
Keywords
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